Cell Types

CD4 Naive/Central memory T cell

This population comprises naive and central memory CD4 T cells characterised by the expression of CCR7, SELL, and LRRN3.

CD4 Effector memory/TEMRA

This subset of CD4 T cells is composed of effector memory cells and cytotoxic cells expressing KLRB1, GZMK, and TNFSF13B. These cells exhibited lower expression levels for CCR7 and SELL compared to the naive/memory compartment.

CD4 SOX4 T cell

CD4 T cells showing a higher expression of SOX4 were categorised in an unconventional group called CD4 SOX4 T cell.

CD8 Naive/Central memory T cell

This population was identified by the expression of CCR7 and SELL, resembling the CD4 Naive/Central memory T cell compartment.

CD8 Effector memory

This cell population was characterised by the expression of cytotoxic and cytolytic markers such as NKG7, GNLY, PFR1. These cells lacked expression of CCR7.

CD8 S100B T cell

This unconventional group overexpressed S100B as opposed to the naive and memory clusters, and was mainly composed of MAIT (Mucosal Associated Invariant T) cells.

Natural Killer Cell

This group was delimited by the expression of NCAM1 and exhibited high expression of cytotoxic and cytolytic markers such as NKG7, GNLY, GZMA, GZMB, and PRF1. Moreover, they considerably lacked expression of CD3* genes as opposed to T cells.

Natural Killer Recruiting Cell

This natural killer population was identified by the overexpression of the chemokine ligands XCL1 and XCL2 when compared to the rest of the natural killer compartment. This cell type resembles the NK CD56 bright phenotype.